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KMID : 0361020220650060309
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Korean Journal of Otolaryngology - Head and Neck Surgery
2022 Volume.65 No. 6 p.309 ~ p.318
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Understanding the Mouse Model of Respiratory Allergic Diseases
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Park Sang-Chul
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Abstract
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Respiratory allergic diseases, represented by allergic rhinitis and asthma, show various fea- tures due to their complex pathophysiology. Mouse models of respiratory allergic diseases replicate the characteristics of human allergic rhinitis and asthma, including airway inflam- mation airway hyperresponsiveness. Different phenotypes and immunologic features are shown according to the various strain of mouse; C57BL/6 strain and BALB/c strain are com- monly used in allergic rhinitis and asthma. Ovalbumin as well as pathophysiologically rele- vant allergen, including house dust mite and fungus are generally used. The mouse model protocols usually include allergen sensitization period followed by allergen provocation peri- od. Allergens possibly combined with adjuvant are introduced into mouse via intraperitoneal, subcutaneous, or intranasal route (sensitization period). Then, mouse repetitively inhales al- lergen by intranasal, intratracheal, or nebulized formation (aerosol), resulting in a robust in- flux of inflammatory cells in the airways (provocation period). We can evaluate several pa- rameters including nasal rubbing and sneezing count, airway hyperresponsiveness, histological assessment, cellular composition, cytokine expression of nasal and lung tissue, and allergen- specific immunoglobulin of serum. These examinations provide useful information to under- stand the pathogenesis of allergic rhinitis and asthma and to test the safety and efficacy of new therapeutic agents. The present review analyzes several protocols and considerations as well as similarities and differences of mouse model of allergic rhinitis and asthma in detail.
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KEYWORD
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Allergic rhinitis, Asthma, Mice
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